|
KMID : 1149220160240040243
|
|
Korean Journal of Oriental Medical Prescription
2016 Volume.24 No. 4 p.243 ~ p.257
|
|
|
Anti-inflammatory Effects of Illicium verum Hook. f. via Suppression of NFκB Pathway
|
|
Keum Soo-Yeon
Park Sang-Mi
Jegal Kyung-Hwan
Hwangbo Min
Cho Il-Je
Park Chung-A
Kim Sang-Chan
Jee Seon-Young
|
|
|
Abstract
|
|
|
Objectives : Illicium verum Hook. f. has been known to possess antimicrobial, antioxidant, antifungal, anti-inflammatory, insecticidal, analgesic, sedative, convulsive activities, it has been rarely conducted to evaluate the immuno-biological activity. The present study was examined to evaluate the anti-inflammatory effects of the Illicium verum Hook. f. water extracts (IVE) in vivo and in vitro.
Methods : Cell viability was measured by MTT assay. The relative levels of NO were measured with Griess reagent. iNOS, COX-2, NF-κB and target proteins were detected by immunoblot analysis, and levels of cytokines were analyzed by ELISA kit. Anti-edema effect was determined in the carrageenan (CA)-induced paw edema model in rats.
Results : All dosages of IVE used in MTT assay had no significant cytotoxicity. The increases of NO production and iNOS expression were detected in LPS-treated cells compared with control. However, these increases were attenuated by treatment with IVE. Also, IVE reduced the elevated production of TNF-α, IL-1β and IL-6 by LPS. IVE inhibited the p-IκB and translocation of NF-κB to nuclear. Furthermore, IVE significantly inhibited the increases of hind paw swelling, skin thicknesses and inflammatory cell infiltrations induced by CA injection. Therefore, IVE will be favorably inhibited the acute edematous inflammations.
Conclusion : These results provide evidences that anti-inflammatory effect of IVE is partly due to the reduction of some inflammatory mediators by suppression of NF-κB pathway.
|
|
|
KEYWORD
|
|
|
Phyllostachys Folium , Anti-inflammatory effects , NF-κB
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|